Structural dependence of ProQ-mRNA interactions in live bacterial cells
Bibliographic record
Abstract
ProQ is a post-transcriptional gene regulator in bacteria known to globally interact with both messenger RNAs (mRNAs) and small regulatory RNAs (sRNAs). Here, we investigate ProQ-mRNA interactions in live bacteria using single-molecule tracking and reveal their dependence on the structural elements of ProQ. We illustrate that during exponential growth phase, stationary phase, and osmotic stress, ProQ mainly binds to mRNAs. All structural domains of ProQ, including N-terminal domain, linker region, and C-terminal domain, contribute to mRNA binding. While residue Y70 is generally required for global mRNA binding, other tested residues in the N-terminal domain have minor to moderate effects on mRNA binding, indicating that different mRNAs likely depend on different ProQ residues for binding. Finally, our data reveal that the expressed sRNA substrates have limited impacts on the fraction of mRNA-associated ProQ, likely due to the high abundance of mRNA-free ProQ inside the cell.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".